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Abstract BackgroundIn ecosystems influenced by strong seasonal variation in insolation, the fitness of diverse taxa depends on seasonal movements to track resources along latitudinal or elevational gradients. Deep pelagic ecosystems, where sunlight is extremely limited, represent Earth’s largest habitable space and yet ecosystem phenology and effective animal movement strategies in these systems are little understood. Sperm whales (Physeter macrocephalus) provide a valuable acoustic window into this world: the echolocation clicks they produce while foraging in the deep sea are the loudest known biological sounds on Earth and convey detailed information about their behavior. MethodsWe analyze seven years of continuous passive acoustic observations from the Central California Current System, using automated methods to identify both presence and demographic information from sperm whale echolocation clicks. By integrating empirical results with individual-level movement simulations, we test hypotheses about the movement strategies underlying sperm whales’ long-distance movements in the Northeast Pacific. ResultsWe detect foraging sperm whales of all demographic groups year-round in the Central California Current System, but also identify significant seasonality in frequency of presence. Among several previously hypothesized movement strategies for this population, empirical acoustic observations most closely match simulated results from a population undertaking a “seasonal resource-tracking migration”, in which individuals move to track moderate seasonal-latitudinal variation in resource availability. DiscussionOur findings provide evidence for seasonal movements in this cryptic top predator of the deep sea. We posit that these seasonal movements are likely driven by tracking of deep-sea resources, based on several lines of evidence: (1) seasonal-latitudinal patterns in foraging sperm whale detection across the Northeast Pacific; (2) lack of demographic variation in seasonality of presence; and (3) the match between simulations of seasonal resource-tracking migration and empirical results. We show that sperm whales likely track oceanographic seasonality in a manner similar to many surface ocean predators, but with dampened seasonal-latitudinal movement patterns. These findings shed light on the drivers of sperm whales’ long-distance movements and the shrouded phenology of the deep-sea ecosystems in which they forage. 

In this paper, we describe the development, testing, and scaling of a leadership development program specifically designed for engineering students and faculty. Such leadership development seeks to catalyze college-wide culture and performance and enhance student success. Program design, content, and process are described. The program combines a system thinking approach, visual models, and principle-based leadership to grow leaders and systems thinking ability concurrently. The program has been tested in small group cohorts, an engineering undergraduate program, and a college-wide co-curricular offering. Our research seeks to assess the effectiveness of the program framework and content as well as the nature and audience of delivery. A process for training faculty is described. 

Among tremendous biodiversity within the California Current Ecosystem (CCE) are gigantic mysticetes (baleen whales) that produce structured sequences of sound described as song. From six years of passive acoustic monitoring within the central CCE we measured seasonal and interannual variations in the occurrence of blue (Balaenoptera musculus), fin (Balaenoptera physalus), and humpback (Megaptera novaeangliae) whale song. Song detection during 11 months of the year defines its prevalence in this foraging habitat and its potential use in behavioral ecology research. Large interannual changes in song occurrence within and between species motivates examination of causality. Humpback whales uniquely exhibited continuous interannual increases, rising from 34% to 76% of days over six years, and we examine multiple hypotheses to explain this exceptional trend. Potential influences of physical factors on detectability – including masking and acoustic propagation – were not supported by analysis of wind data or modeling of acoustic transmission loss. Potential influences of changes in local population abundance, site fidelity, or migration timing were supported for two of the interannual increases in song detection, based on extensive local photo ID data (17,356 IDs of 2,407 individuals). Potential influences of changes in foraging ecology and efficiency were supported across all years by analyses of the abundance and composition of forage species. Following detrimental food web impacts of a major marine heatwave that peaked during the first year of the study, foraging conditions consistently improved for humpback whales in the context of their exceptional prey-switching capacity. Stable isotope data from humpback and blue whale biopsy samples are consistent with observed interannual variations in the regional abundance and composition of forage species. This study thus indicates that major interannual changes in detection of baleen whale song may reflect underlying variations in forage species availability driven by energetic variations in ecosystem state. 

Rationale Early life social rearing has profound consequences on offspring behavior and resilience. Yet, most studies examining early life development in rodents use species whose young are born immobile and do not produce complex social behavior until later in development. Furthermore, models of rearing under increased social complexity, rather than deprivation, are needed to provide alternative insight into the development of social neural circuitry. Objectives To understand precocial offspring social development, we manipulated early life social complexity in the communal spiny mouse Acomys cahirinus and assessed long-term consequences on offspring social behavior, exploration, and neural responses to novel social stimuli. Methods Spiny mouse pups were raised in the presence or absence of a non-kin breeding group. Upon adulthood, subjects underwent social interaction tests, an open field test, and a novel object test. Subjects were then exposed to a novel conspecific and novel group and neural responses were quantified via immunohistochemical staining in brain regions associated with social behavior. Results Early life social experience did not influence behavior in the test battery, but it did influence social processing. In animals exposed to non-kin during development, adult lateral septal neural responses toward a novel conspecific were weaker and hypothalamic neural responses toward a mixed-sex group were stronger. Conclusions Communal species may exhibit robust behavioral resilience to the early life social environment. But the early life environment can affect how novel social information is processed in the brain during adulthood, with long-term consequences that are likely to shape their behavioral trajectory. 

Isotope ratio analyses of trace elements are applied to tooth enamel, ostrich eggshell, and other archaeological hard tissues to infer mobility and other aspects of hominin and animal paleoecology. It has been assumed that these highly mineralized tissues are resistant to diagenetic alteration, but this is seldom tested and some studies document diagenetic alteration over brief time spans. Here, we build on existing research on Maximum Threshold Concentrations (MTCs) to develop screening tools for diagenesis that can inform heavy isotopic analyses. The premise of the MTC approach is that archaeological tissues are likely contaminated and unsuitable for isotope ratio analysis when they exceed characteristic modern concentration ranges of trace elements. Furthermore, we propose a new metric called the Maximum Threshold Ratio (MTR) of 85Rb/88Sr or whole element Rb/Sr, which can be measured simultaneously with 87Sr/86Sr during laser ablation (LA) MC-ICP-MS or applied during post hoc screening of specimens. We analyzed 56 enamel samples from modern Kenyan mammals and 34 modern ostrich eggshells from South Africa, Namibia, and the United States by solution ICP-MS, as well as a subset of shells using LA-MC-ICP-MS. Our results indicate that thresholds are consistent across taxa at a single location, but likely vary across locations. Therefore, MTCs and MTRs need to be tissue and locality specific, but not necessarily taxon-specific. Other important differences are observed between the inner and outer surfaces of the eggshells and between LA and solution ICP-MS. This exploratory study provides guidelines for building reference thresholds to screen enamel and eggshell for diagenesis potentially impacting biogenic isotope ratios. 

The fighting fish Betta splendens, long studied for its aggressive territorial competitions, has the potential to be a tractable and relevant model for studying the intersection of cognitive ecology and social neuroscience. Yet, few studies have comprehensively assessed Betta behavior across both social and nonsocial contexts. Furthermore, the present study is the first to quantify the expression of phosphorylated ribosomal protein S6 (PS6), a proxy for neural response, in the Betta telencephalon. Here, we assessed male Betta behavior across a suite of tasks and found that response to a mirror, but not neophilia (a novel object) nor anxiety (scototaxis), predicted behavior in a social competition. To then explore the cognitive aspects of social competition, we exposed Betta to either a familiar or novel opponent and compared their competitive behavior as well as their neural responses in the teleost homologs of the hippocampus, basolateral amygdala, and lateral septum. We did not detect any differences between familiar-exposed and novel-exposed individuals, but by implementing the first use of a habituation–dishabituation competition design in a study of Betta, we were able to observe remarkable consistency in competitive outcomes across repeated exposures. Taken together, the present study lays the groundwork for expanding the use of Betta to explore integrative and multidimensional questions of social cognition. 

Abstract Hellbenders ( Cryptobranchus alleganiensis ) are large, aquatic salamanders from the eastern United States. Both subspecies, eastern and Ozark hellbenders, have experienced declines resulting in federal listing of Ozark hellbenders. The globally distributed chytrid fungus, Batrachochytrium dendrobatidis (Bd) has been detected in both subspecies, and Batrachochytrium salamandrivorans ( Bsal ) poses a new threat if introduced into North America. Ozark hellbenders also suffer a high prevalence of toe lesions of unknown etiology, with changes in host immunocompetence hypothesized to contribute. Antimicrobial peptides (AMPs) secreted from dermal granular glands may play a role in hellbender health. We collected skin secretions from free-ranging hellbenders and enriched them for small cationic peptides used for growth inhibition assays against Bd and Bsal . Generalized linear mixed models revealed the presence of active toe lesions as the strongest and only significant predictor of decreased Bd inhibition by skin peptides. We also found skin secretions were more inhibitory of Bsal than Bd . MALDI-TOF mass spectrometry revealed candidate peptides responsible for anti-chytrid activity. Results support the hypothesis that hellbender skin secretions are important for innate immunity against chytrid pathogens, and decreased production or release of skin peptides may be linked to other sub-lethal effects of disease associated with toe lesions. 

Strain engineering in two-dimensional (2D) materials is a powerful but difficult to control approach to tailor material properties. Across applications, there is a need for device-compatible techniques to design strain within 2D materials. This work explores how process-induced strain engineering, commonly used by the semiconductor industry to enhance transistor performance, can be used to pattern complex strain profiles in monolayer MoS2 and 2D heterostructures. A traction–separation model is identified to predict strain profiles and extract the interfacial traction coefficient of 1.3 ± 0.7 MPa/μm and the damage initiation threshold of 16 ± 5 nm. This work demonstrates the utility to (1) spatially pattern the optical band gap with a tuning rate of 91 ± 1 meV/% strain and (2) induce interlayer heterostrain in MoS2–WSe2 heterobilayers. These results provide a CMOS-compatible approach to design complex strain patterns in 2D materials with important applications in 2D heterogeneous integration into CMOS technologies, moiré engineering, and confining quantum systems. 

Efforts dedicated towards broadening participation for Black and other underrepresented groups in engineering at post-secondary institutions has intensified in recent decades. However, Black women have not yet reached parity in undergraduate engineering degree attainment. To elucidate this trend, data from the U.S. Department of Education was analyzed to investigate postsecondary completion for Black women in engineering. Results indicate that the percentage of degrees awarded to Black women has slightly decreased during the last five years when compared to women of all races. However, the percentage of Black women obtaining engineering degrees has increased when compared to the general Black engineering population, with a larger percentage of Black women obtaining engineering degrees compared to their male counterparts than any other ethnicity. Thus, the purpose of this paper is to provide recommendations for research avenues that may strengthen knowledge around the enrollment and retention of Black women in engineering at post-secondary institutions.